Madge - Microplate Array Diagonal Gel Electrophoresis
نویسندگان
چکیده
منابع مشابه
Typing dinucleotide repeat loci using microplate array diagonal gel electrophoresis: proof of principle.
Polymorphic dinucleotide repeat loci ('microsatellite markers') are found in varying abundance throughout the genomes of most organisms. They have been extensively used for genetic studies, but conventional techniques used for their genotyping require sophisticated equipment. Microplate array diagonal gel electrophoresis (MADGE) has previously been extended to economical high-throughput genotyp...
متن کاملMicroplate array diagonal gel electrophoresis for cohort studies of microsatellite loci.
After PCR amplification, we have achieved precise sizing of trinucleotide and tetranucleotide microsatellite alleles on 96-well open-faced polyacrylamide microplate array diagonal gel electrophoresis (MADGE) gels: two tetranucleotide repeats, HUMTHOI (five alleles 248-263 bp) and DYS390 (eight alleles 200-228 bp), and DYS392, a trinucleotide repeat (eight alleles 210-231 bp). A gel matrix of Du...
متن کاملCombination of His-tagged T4 endonuclease VII with microplate array diagonal gel electrophoresis for high-throughput mutation scanning.
fied in the stalk region. J Biol Chem 2001;276:5525–32. 21. Okabe T, Fujisawa M, Yotsumoto H, Watanabe J, Takaku F, Lanzillo JJ, et al. Familial elevation of serum angiotensin-converting enzyme. Q J Med 1985; 55:55–61. 22. Luisetti M, Martinetti M, Cuccia M, Dugoujon M, De Rose V, Peona V, et al. Familial elevation of serum angiotensin-converting enzyme activity. Eur Resp J 1990;3:441–6. 23. Kr...
متن کاملHigh-throughput method for determination of apolipoprotein E genotypes with use of restriction digestion analysis by microplate array diagonal gel electrophoresis.
Molecular epidemiological research has identified the association of a common apolipoprotein E (apo E) isoform (E4 as opposed to E3), with risk both of coronary artery disease and of Alzheimer dementia. In addition, the role of apo E genotype (usually E2/E2) in Type III hyperlipidemia is well known. However, both for diagnostic and research purposes, apo E genotyping is cumbersome. The preferre...
متن کاملSNP genotyping by combination of 192-well MADGE, ARMS and computerized gel image analysis.
A new modification of the microplate array diagonal gel electrophoresis (MADGE) system accommodates the dual amplification refractory mutation system (ARMS) products of 96 samples on one 192-well gel. Simultaneous electrophoresis of a number of horizontal ARMS-MADGE gels achieves high throughput. Gels are imaged digitally, here using the FluorImager 595 fluorescent scanning system. Customized s...
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ژورنال
عنوان ژورنال: Journal of Medical Biochemistry
سال: 2009
ISSN: 1452-8266,1452-8258
DOI: 10.2478/v10011-009-0030-y